A restriction enzyme is a DNA-cutting enzyme that recognizes specific sites in DNA. Many restriction enzymes make staggered cuts at or near their recognition sites, producing ends …

Restriction digestion. Sticky ends and blunt ends. Ligation reactions.

Where they come from, why they have such strange names, and more. This article reviews the history and nomenclature of restriction enzymes - where they come from and why they have …

Thus, when they figured out what enzyme is causing that, they basically called it a restriction enzyme, 'cause it restricted the growth of viruses. Now, in order for the restriction enzyme to …

DNA cloning involves creating identical copies of a specific gene using restriction enzymes to cut the desired gene, pasting it into a plasmid, and inserting it into bacteria like E. coli. The …

Let's explore why cloning vectors need to have a few cloning sites for every restriction enzyme, but multiple cloning sites for multiple enzymes. We will also explore why the same restriction …

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Is there a way to cut DNA into fragments? Are there any "scissors" for that? How would they work? Watch this video to understand more about restriction enzymes aka molecular scissors, …

A restriction enzyme is a DNA-cutting enzyme that recognizes a specific target sequence and cuts DNA into two pieces at or near that site. Many restriction enzymes produce cut ends with …

See the article on restriction enzymes and DNA ligase for a more concrete example of how and why these different ligation products can form.