Oxidoreductases are key enzymes in biocatalysis, but their dependence on the cofactor nicotinamide adenine dinucleotide (phosphate) (NAD(P)) presents challenges due to its high consumption and ...

Cytochrome P450 monooxygenases are widely involved in the synthesis and metabolism of endogenous and exogenous substances in living organisms. The catalytic efficiency of cytochrome P450 monooxygenase ...

A peptide bridge is designed to construct a fusion enzyme with electrostatic cofactor channeling, reducing NADPH input by two orders of magnitude or decreasing reaction time threefold with the same ...

Kinetic aspects of enzymatic reactions are described by equations based on the Michaelis–Menten theory for the initial stage. However, the kinetic parameters provide little information on the atomic ...

Biomanufacturing of renewable chemicals by engineered microbes 1,2 or synthetic biochemistry 3 has the potential to supplant many petroleum-derived chemical industries 4. However, many engineered ...